ISSN 1392-3196 / e-ISSN 2335-8947
Zemdirbyste-Agriculture, vol. 100, No. 3 (2013), p. 317–324
DOI  10.13080/z-a.2013.100.041

Comparison of spring barley (Hordeum vulgare L.) screening methods for Fusarium head blight resistance breeding



Fusarium head blight (FHB) is a widespread disease of cereals, including barley (Hordeum vulgare L.), that causes substantial grain yield losses and grain quality deterioration. Resistant varieties provide the greatest potential for reducing FHB. In the present study, we adapted two methods for screening FHB severity using Fusarium culmorum. We used a cut-spike method and a spray inoculation method in the field conditions for screening different spring barley genotypes of Latvian and foreign origin. Screening with the cut-spike method was done for 126 different genotypes in 2008 and in 2009 and for 180 genotypes in 2010. In the years 2011 and 2012, the field inoculation method was used to screen 207 and 164 genotypes, respectively; part of material was tested simultaneously by both methods. A significant effect of genotype was found for both cut-spike and field inoculation methods (p < 0.001); field inoculation method showed a greater proportion of genotype effect and a higher heritability. Using area under the disease progress curve (AUDPC) and score obtained at 26 days after inoculation were equally effective. Correlations between results obtained by both methods in different years were not significant, though, the data of the same year correlated significantly (r = 0.36–0.47, p < 0.05). Results suggest that differences between breeding material can be assessed by both methods; however, the information obtained was contradictory for a number of genotypes. Considering the results and the fact that field inoculation method can more likely provide results of equal value to those in natural conditions in respect to identification of resistant and susceptible genotypes, field inoculation method was recognized as preferable for the assessment of spring barley breeding material. Significant correlation (r = 0.71, p < 0.05) between visual estimate of disease severity and mycotoxin deoxynivalenol (DON) concentration in grain was found in 2011, but in the environmental conditions of 2012 formation of DON was inhibited.

Key words: cut-spike method, DON, field inoculation method, Fusarium culmorum, genotype effect.

Full text:  100_3_str41.pdf