ISSN 1392-3196 / e-ISSN 2335-8947
Zemdirbyste-Agriculture, vol. 101, No. 4 (2014), p. 367–372
DOI  10.13080/z-a.2014.101.046

The content of carbon, nitrogen, phosphorus and sulphur in soil against the activity of selected hydrolases as affected by crop rotation and fertilisation



The aim of the paper was to determine the effect of crop rotation and different fertilisation with manure and nitrogen on the content of phosphorus and sulphur against the activity of phosphatase and arylsulphatase. The soil was sampled from a long-term field experiment carried out in a three-factor design, which covered two crop rotation types (A – enriching and B – depleting the soil from organic matter), manure fertilisation (0, 20 and 60 t ha-1) and nitrogen fertilisation (N0, N1 and N2). The experiment was performed on a Haplic Luvisol (LVha). The soil was sampled in each year during a 4-year (2009–2012) rotation. There was found a significant effect of the experiment factors on the content of available forms of the elements. According to the criteria provided for in Chemical and Agricultural analysis – determination of the content of available phosphorus in mineral soils (PN-R-04023:1996) the soil represented the second highest class of richness with available phosphorus. The activity of the hydrolytic enzymes depended both on the application of manure and ammonium nitrate. The synthesis of the results demonstrated a significant effect of the type of the crop rotation on the content of total organic carbon, total nitrogen, available phosphorus and sulphur considering a selected hydrolases activity. Growing crops in a crop rotation enriching the soil with organic matter increased the richness of the soil with available forms of phosphorus and sulphur and increased the activity of acid phosphatase and arylsulphatase.

Key words: alkaline and acid phosphatases, arylsulphatase, crop rotation, fertilization, phosphorus, sulphur.

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