105(3)_str29

ISSN 1392-3196 / e-ISSN 2335-8947
Zemdirbyste-Agriculture, vol. 105, No. 3 (2018), p. 227–234
DOI  10.13080/z-a.2018.105.029

The effect of a growth regulator Ribav-Extra on winter wheat seedlings exposed to heavy metals

Kristina A. GRUZNOVA, Dmitry I. BASHMAKOV, Jurga MILIAUSKIENĖ, Viktorija VAŠTAKAITĖ, Pavelas DUCHOVSKIS, Alexander S. LUKATKIN

Abstract

We studied the influence of a natural plant growth regulator Ribav-Extra and ions of heavy metals (HM) Pb²⁺, Cu²⁺, Zn²⁺ and Ni²⁺ on the physiological and biochemical indices of the winter wheat (Triticum aestivum L. ) cultivar ‘Mironovskaya 808’. The seeds of wheat were treated with Ribav-Extra (10 ppm) and grown for 7 days on heavy metal salt solutions (10 μM or 1 mM). After that we recorded heavy metals accumulation, growth, generation of superoxide anion (^•O₂⁻), lipid peroxidation (LPO), and catalase (CAT) and ascorbate peroxidase (APOX) activity. It was found that 1 mM of HM in growth media increased heavy metals accumulation in wheat plants and inhibited the growth of roots and shoots. Both low and high concentrations of heavy metals stimulated ^•O₂⁻ production and oxidative damage in wheat seedlings. In most treatments, the toxic effect of heavy metals enhanced at higher concentration of metals. Pre-sowing treatment of seeds with Ribav-Extra decreased the negative impact of heavy metals by reducing oxidative stress which led to suppressed ^•O₂⁻ generation, lipid peroxidation intensity and catalase activity. This suggests that the wheat plants, whose seeds had been treated with Ribav-Extra, were more heavy metal-resistant than the untreated ones.

Key words: antioxidative enzymes, heavy metals, oxidative stress, Ribav-Extra, Triticum aestivum.

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